Ottawa Police say they arrested one person during the fifth wave of a national human trafficking investigation.
OPP say over six days, 36 services in Ontario played a part in taking 25 people into custody on 67 offences.
32 arrests were made across the country.
The suspect arrested by Ottawa Police faces charges of possession of marijuana and possession of a dangerous weapon.
Officers contacted 10 sex trade workers in the city.
Charges laid across Operation Northern Spotlight include human trafficking, making and distributing child pornography, child luring, assault and many others.
OPP say the joint effort was able to ensure the safety of 16 people who had been working in the sex trade, either as a minor or against their will.